REPEATED LOW-LEVEL EXPOSURE OF THE ROUND GOBY (NEOGOBIUS MELANOSTOMAS) TO CLOSTRIDIUM BOTULINUM TYPE E NEUROTOXIN

Cloning, Expression and Purification of Clostridium botulinum Neurotoxin Type E Binding Domain

Toxicity of Clostridium botulinum type E neurotoxin to Great Lakes fish: implications for avian botulism.

Since 1999, large-scale mortalities of fish-eating birds have been observed on the Great Lakes, and more specifically on Lake Erie. Type E botulism has been established as the primary cause of death. The mechanism of type E botulism exposure in fish-eating birds is unclear. Given that these birds are thought to eat live fish exclusively, it seems likely that their prey play a key role in the pr...

Purification of Clostridium botulinum type A neurotoxin.

Acoustic communication modalities of the round goby ( Neogobius melanostomus)

Acoustic communication is essential to survival for many fish species, however it is often understudied. This thesis investigates two areas of acoustic communication in the round goby (Neogobius melanostomus). These fish have no accessory hearing structures, and theory would predict they would be unable to localize sound sources, however it has been shown that round gobies can localize conspeci...

Immunogenic and Protective Potentials of Recombinant Receptor Binding Domain and a C-Terminal Fragment of Clostridium botulinum Neurotoxin Type E

Clostridium Botulinum Type E neurotoxin heavy chain consists of two domains: the translocation domain asthe N-terminal half and the binding domain as the Cterminal half (Hc). One effective way to neutralize botulinum neurotoxin is to inhibit binding of this toxin to neuromuscular synapses with antibodies against binding domain. Two synthetic genes, coding for Hc (the full length binding d...